Comparison of the exoS Gene and Protein Expression in Soil and Clinical Isolates of Pseudomonas aeruginosa

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Silva Jack da (Creator)

Michael W. Ferguson (Creator)

Jill A. Maxwell (Creator)

Joan C. Olson (Creator)

Timothy S. Vincent (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Exoenzyme S (ExoS) is translocated into eukaryotic cells by the type III secretory process and has been hypothesized to function in conjunction with other virulence factors in the pathogenesis of Pseudomonas aeruginosa. To gain further understanding of how ExoS might contribute to P. aeruginosa survival and virulence ExoS expression and the structural gene sequence were determined in P. aeruginosa soil isolates and compared with ExoS of clinical isolates. Significantly higher levels of ExoS ADP-ribosyltransferase (ADPRT) activity were detected in culture supernatants of soil isolates compared to those of clinical isolates. The higher levels of ADPRT activity of soil isolates reflected both the increased production of ExoS and the production of ExoS having a higher specific activity. ExoS structural gene sequence comparisons found the gene to be highly conserved among soil and clinical isolates with the greatest number of nonsynonymous substitutions occurring within the region of ExoS encoding GAP function. The lack of amino acid changes in the ADPRT region in association with a higher specific activity implies that other factors produced by P. aeruginosa or residues outside the ADPRT region are affecting ExoS ADPRT activity. The data are consistent with ExoS being integral to P. aeruginosa survival in the soil and suggest that in the transition of P. aeruginosa from the soil to certain clinical settings the loss of ExoS expression is favored. Originally published Infection and Immunity Vol. 69 No. 4 Apr 2001

Additional Information

Publication

Other

Infection and Immunity. 69:4(April 2001) p. 2198-2210.

Language: English

Date: 2011

Keywords

Peudomonas aeruginosa, Exoenzyme S, Gene expression

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