Construction Of A [delta]vpsL[delta]potB Mutant And The Effects On Biofilm Formation In Vibrio cholerae

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Mackenzie Brown (Creator)
Appalachian State University (ASU )
Web Site:
Ece Karatan

Abstract: Vibrio cholerae is a Gam-negative, pathogenic bacterium known as the causative agent of the intestinal disease cholera. Infection is linked to ingestion of contaminated water. Four hundred million people worldwide are without safe or up to standard drinking water, making them more prone to experience fatalities and symptoms associated with the disease. V. cholerae utilizes its ability to form biofilms, aggregations of microorganisms, to survive environmental stressors. These biofilms enable the bacterium further resistance and survival abilities and is thought to be the form the bacterium is ingestion in. While cholera can be treated through adequate rehydration, these rehydration practices are generally linked to the contamination sources themselves. V. cholerae continues to adapt new variant strains that allow it to survive further stressors and immunity efforts. Construction of a ?vpsL?potB mutant allowed further manipulation of the numerous genes that constitute the bacterium’s ability to form biofilms. The bacterium is composed of numerous aspects that lead to its ability to form these mature structures, with some being able to compensate for the loss of others. Therefore, understanding these aspects in full would allow for better and more targeting treatment plans.

Additional Information

Honors Project
Brown, M. (2018). "Construction Of A [delta]vpsL[delta]potB Mutant And The Effects On Biofilm Formation In Vibrio cholerae." Unpublished Honors Thesis. Appalachian State University, Boone, NC.
Language: English
Date: 2018
Vibrio cholerae, Cholera, Biofilm

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