Anti-tumor natural product research focused on plants found in the southern Appalachian region
- WCU Author/Contributor (non-WCU co-authors, if there are any, appear on document)
- Matthew John Flood II (Creator)
- Institution
- Western Carolina University (WCU )
- Web Site: http://library.wcu.edu/
- Advisor
- Jason Clement
Abstract: Natural products have been an integral part of society since the beginning of recorded
history. Various types of herbs, plants, and even marine organisms have been used as
both topical and ingestible remedies for a variety of health concerns and problems
throughout the years. Our research efforts focus on the isolation and characterization of
antitumor compounds from plant extracts from western North Carolina, a surprisingly
diverse and under studied region of the country. Recent work has been done on the plant
species Aralia racemosa and Arnoglossum atriplicifolium. Crude extracts were initially
separated using either a modified Kupchan Partitioning or standard acid/base extraction,
and then further purified using various chromatographic techniques. Two compounds
resulted from this work on the two species and were characterized using various methods
of mass spectrometry, and advanced NMR experiments including COSY, HMBC, and
HSQC. An ent-kaurane diterpenoid was isolated from Aralia racemosa, and a highly
oxygenated oplopane derivative from Arnoglossum atriplicifolium. The activity of the
compounds was determined through a MCF-7 assay (breast cancer), with the control
group being a FS-4 assay, both carried out by Wake Forest University. This is the first
report of the antitumor activity of each of these compounds.
Anti-tumor natural product research focused on plants found in the southern Appalachian region
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Created on 8/1/2010
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Additional Information
- Publication
- Thesis
- Language: English
- Date: 2010
- Keywords
- Anti-tumor, Appalachian, Aralia racemosa, Arnoglossum atriplicifolia, Natural Products
- Subjects
- Materia medica, Vegetable -- Appalachian Region, Southern
- Materia medica, Vegetable -- Testing
- Botany, Medical -- Appalachian Region, Southern
- Araliaceae -- Therapeutic use
- Compositae -- Therapeutic use