Characteristics and utility of plastid-encoded 16S rRNA gene sequence data in phylogenetic studies of red algae
- UNCW Author/Contributor (non-UNCW co-authors, if there are any, appear on document)
- Wilson Freshwater, Research Specialist (Creator)
- Institution
- The University of North Carolina Wilmington (UNCW )
- Web Site: http://library.uncw.edu/
Abstract: A set of oligonucleotide primers was designed for amplifying and sequencing the plastid-encoded 16S rRNA gene from red algae. Gene sequences (16S rRNA) were generated from 28 species representing 11 red algal orders. Alignment of sequences from a wide taxonomic sampling required only a small number of gapped sites and revealed distinct variable and conserved regions in the gene. Likewise, a more narrow taxonomic sampling of 15 sequences from Gelidiales species was analyzed and compared with complementary sequence data sets for the nuclear-encoded 18S rRNA and 28S rRNA genes and plastid-encoded rbcL. Alignment of the Gelidiales 16S rRNA gene sequences also required only a small number of gapped sites and the resulting data set contained both highly conserved and variable regions. The amount of variation in 16S rRNA gene sequences was similar to that found in the 28S rRNA gene, and intermediate between the level of variation in rbcL and the 18S rRNA gene when complementary data sets were compared. Phylogenetic analyses of 16S rRNA gene sequences should be a useful tool for researchers studying the evolution, systematics and taxonomy of red algae.
Characteristics and utility of plastid-encoded 16S rRNA gene sequence data in phylogenetic studies of red algae
PDF (Portable Document Format)
525 KB
Created on 7/3/2013
Views: 1506
Additional Information
- Publication
- Olson, K.N., R.S. Melton, K.M. Yaudes, K.G. Norwood & D.W. Freshwater. 2004. Characteristics and utility of plastid-encoded 16S rRNA gene sequence data in phylogenetic studies of red algae. Journal of the North Carolina Academy of Sciences 120:143-151.
- Language: English
- Date: 2004
- Keywords
- cp 16S rRNA, Gelidiales, Phylogenetic analyses, Red algae
- Subjects
- Red algae
- Marine algae
- Gelidiales
- Plants—Phylogeny
- Biology—Classification—Molecular aspects