Characterization of bioactive secondary metabolites from Pseudomonas aeruginosa and Prorocentrum species

UNCW Author/Contributor (non-UNCW co-authors, if there are any, appear on document)
Jennifer S. Williams (Creator)
The University of North Carolina Wilmington (UNCW )
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Abstract: Environmental isolates of Pseudomonas aeruginosa, Prorocentrum maculosum and Prorocentrum lima, organisms that have an adverse impact on human health, were mined for bioactive secondary metabolites. Antibiotics isolated from the extracts of P. aeruginosa culture supernatant included pyoluteorin, phenazine-1-carboxamide, and five members of the 4-hydroxy-2-alkyl/alkenyl quinolines. Several diarrhetic shellfish poisons (DSPs) including okadaic acid, dinophysis toxin-1 and two new methyl derivatives of okadaic acid were isolated from the Prorocentrum species’ cell-free culture media, while three diol esters of okadaic acid and one of DTX-1 were isolated from P. lima cell extracts. Isolation of the DSPs was facilitated by a new, efficient method designed during this investigation. Algal as well as bacterial isolates from this study were tested in a B-galactosidase bioassay designed to measure induction or inhibition of P. aeruginosa cell-to-cell signaling. The P. aeruginosa cell-signaling cascade was induced by select P. aeruginosa and P. lima extracts, however the presence of known P. aeruginosa autoinducers was not detected. OA and DTX-1 were found to have no significant impact on the cell-signaling cascade of the bioassay strain.

Additional Information

A Thesis Submitted to the University of North Carolina Wilmington in Partial Fulfillment of the Requirements for the Degree of Master of Science
Language: English
Date: 2009
Metabolites--Environmental aspects--United States, Metabolites--United States--Analysis, Pseudomonas aeruginosa
Pseudomonas aeruginosa
Metabolites -- Environmental aspects -- United States
Metabolites -- United States -- Analysis

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