Characterization of bioactive secondary metabolites from Pseudomonas aeruginosa and Prorocentrum species
- UNCW Author/Contributor (non-UNCW co-authors, if there are any, appear on document)
- Jennifer S. Williams (Creator)
- Institution
- The University of North Carolina Wilmington (UNCW )
- Web Site: http://library.uncw.edu/
Abstract: Environmental isolates of Pseudomonas aeruginosa, Prorocentrum maculosum
and Prorocentrum lima, organisms that have an adverse impact on human health, were
mined for bioactive secondary metabolites. Antibiotics isolated from the extracts of P.
aeruginosa culture supernatant included pyoluteorin, phenazine-1-carboxamide, and five
members of the 4-hydroxy-2-alkyl/alkenyl quinolines. Several diarrhetic shellfish
poisons (DSPs) including okadaic acid, dinophysis toxin-1 and two new methyl
derivatives of okadaic acid were isolated from the Prorocentrum species’ cell-free culture
media, while three diol esters of okadaic acid and one of DTX-1 were isolated from P.
lima cell extracts. Isolation of the DSPs was facilitated by a new, efficient method
designed during this investigation. Algal as well as bacterial isolates from this study
were tested in a B-galactosidase bioassay designed to measure induction or inhibition of
P. aeruginosa cell-to-cell signaling. The P. aeruginosa cell-signaling cascade was
induced by select P. aeruginosa and P. lima extracts, however the presence of known P.
aeruginosa autoinducers was not detected. OA and DTX-1 were found to have no
significant impact on the cell-signaling cascade of the bioassay strain.
Characterization of bioactive secondary metabolites from Pseudomonas aeruginosa and Prorocentrum species
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Created on 1/1/2009
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Additional Information
- Publication
- Thesis
- A Thesis Submitted to the University of North Carolina Wilmington in Partial Fulfillment of the Requirements for the Degree of Master of Science
- Language: English
- Date: 2009
- Keywords
- Metabolites--Environmental aspects--United States, Metabolites--United States--Analysis, Pseudomonas aeruginosa
- Subjects
- Pseudomonas aeruginosa
- Metabolites -- Environmental aspects -- United States
- Metabolites -- United States -- Analysis