Determination of single and competitive binary absorption isotherms for R and S optical isomers of fluoxetine

UNCW Author/Contributor (non-UNCW co-authors, if there are any, appear on document)
Jennifer L Beddoe (Creator)
Institution
The University of North Carolina Wilmington (UNCW )
Web Site: http://library.uncw.edu/
Advisor
Dean Shirazi

Abstract: The pharmaceutical industry has come to rely on isotherm data when working with preparative chromatography. The data derived during isotherm measurement is used to optimize experimental conditions which will be used in the making of drugs. For the R- and S- enantiomers of Fluoxetine, four different methods were used to determine the single component isotherms and this data was fitted to the Langmuir Isotherm Model. Both enantiomers, when tested singly, fit the model using the Frontal Analysis Method and Retention Time Method, but did not fit the model when using the FACP and ECP methods. The major objective of this research was to determine whether the single component isotherm measurement can be used to predict the multi-component isotherm in order to avoid the cumbersome, tedious and very time consuming procedures for measuring the multi-component isotherm. Prediction of a multi-component isotherm using single component isotherms is in most cases impossible due to the competition between the two solutes and the difference in column saturation capacity of the two compounds. The two components of a chiral compound have very similar physical properties and therefore they should have similar column saturation capacities. The competitive binary isotherm was determined on the racemic mixture of Fluoxetine using the Frontal Analysis Method for Multi-Component Isotherms. It was then compared to values predicted from the Single Component studies and it was determined that the Multi-Component isotherm could be predicted from data obtained from the isotherms of the single components.

Additional Information

Publication
Thesis
A Thesis Submitted to the University of North Carolina at Wilmington in Partial Fulfillment of the Requirement for the Degree of Masters of Science
Language: English
Date: 2009
Keywords
Fluoxetine--Therapeutic use, Fluoxetine--Analysis, Gas chromatography
Subjects
Fluoxetine -- Therapeutic use
Gas chromatography
Fluoxetine -- Analysis