A psbA phylogeny for selected rhodophyceae

UNCW Author/Contributor (non-UNCW co-authors, if there are any, appear on document)
Jannine M. Hunt (Creator)
The University of North Carolina Wilmington (UNCW )
Web Site: http://library.uncw.edu/
J. Craig Bailey

Abstract: Oligonucleotide primers for PCR amplifying and sequencing the plastid-encoded psbA gene, which encodes the D1 protein of photosystem II, were developed for red algae. Using these primers psbA sequences were determined for 18 different species representing 10 phylogenetically diverse red algal orders. The third codon position of the psbA gene is too variable (= homoplasious, mutationally saturated) to be used to estimate phylogenetic relationships among the orders investigated here, whereas amino acid sequences are too conserved. In contrast, analyses including only first and second codon positions yielded trees for red algae that are, with exceptions, congruent with those obtained for other genes. Comparisons of sequence divergence values and phylogenetic measures imply that psbA sequences are best suited for use at the rank of family and below.

Additional Information

A Thesis Submitted to the University of North Carolina at Wilmington in Partial Fulfillment of the Requirement for the Degree of Masters of Science
Language: English
Date: 2009
Gene mapping, Red algae--Genetics, Red algae--Molecular genetics, Red algae--Phylogeny--Molecular aspects
Red algae -- Molecular genetics
Gene mapping
Red algae -- Phylogeny -- Molecular aspects
Red algae -- Genetics

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