Phosphorus and carbohydrate limtation [i.e. limitation] of fecal coliform and fecal enterococcus within tidal creek sediments
- UNCW Author/Contributor (non-UNCW co-authors, if there are any, appear on document)
- Byron R. Toothman (Creator)
- Institution
- The University of North Carolina Wilmington (UNCW )
- Web Site: http://library.uncw.edu/
- Advisor
- Lawrence Cahoon
Abstract: Aquatic sediments have been shown to be a significant reservoir for fecal bacteria and at
concentations two to three orders of magnitude higher than the waters directly above them.
These bacteria represent a potentially serious health threat to humans using these waters. This
study was conducted to determine the abundance of fecal bacteria within tidal creek sediments of
Bradley Creek and determine if their residence or growth may be limited by concentrations of
sediment phosphorus (P), sediment carbon (C), salinity, and water temperature. Sediment fecal
coliforms had a mean of 179 CFU/cm2 (std. dev. = 411, range = 0-3230) for samples collected
monthly at 6 stations over the course of this study. Were the bacteria and sediments to be
suspended through a 100 cm water column this value would be the equivalent of 179 CFU/100ml
which is just below the water quality standard for human contact (200 CFU/100ml). Such
disturbances could easily be produced by natural or human activities. Samples for enterococcus
had a mean of 285 CFU/cm2 (std. dev. = 473, range = 0-1730). If these sediments and bacteria
were similarly suspended they would equate to 285 CFU/100ml and greatly exceede the standard
for human contact in the water column (33 CFU/100ml). Overall, only fecal coliform bacteria
were correlated to sediment C, however, the signal from bioavailable C was probably masked by
the presence of insoluble C from detrital cellulose. Neither bacteria were correlated to sediment
P concentrations which were found to be greater in Bradley Creek sediments than limiting
concentrations for coliforms in sediments concluded by previous research. Sediments were a
significant reservoir of P as concentrations were recorded as high as 4-5 orders of magnitude
greater than in overlying waters. Sediment fecal coliforms were shown to be negatively
correlated with salinity and positively correlated with temperature conforming to patterns
established by previous research. However, fecal enterococcus was not shown to have a significant relationship with either salinity or temperature. Fecal coliforms were positively
correlated to precipitation over the previous 24 hours.
Experimental addition of bioavailable P (potassium phosphate monobasic) and
bioavailable C (dextrose) showed a positive relationship between both fecal bacteria and
bioavailable C. Enterococcus was significantly correlated to P in trials with low initial sediment
P concentrations. Fecal coliform was significantly correlated to P at a = 0.1 where initial P
concentrations were low. A higher a was taken into consideration due to the high variability of
coliform data and relatively low degrees of freedom for individual experimental trials.
It was concluded that while P and C are important to fecal bacterial residence within
sediments, P may no longer be limiting in Bradley Creek due to relatively high background
concentrations. Elevated P and bioavailable C concentrations have been correlated to storm
water runoff. Limitation of sediment fecal bacteria in Bradley Creek by these nutrients may be
alleviated from their introduction via this mechanism.
Phosphorus and carbohydrate limtation [i.e. limitation] of fecal coliform and fecal enterococcus within tidal creek sediments
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Created on 1/1/2009
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Additional Information
- Publication
- Thesis
- A Thesis Submitted to the University of North Carolina at Wilmington in Partial Fulfillment of the Requirement for the Degree of Masters of Science
- Language: English
- Date: 2009
- Keywords
- Bacterial pollution of water--North Carolina--Bradley Creek, Enterococcus--Environmental aspects--North Carolina--Bradley Creek, Marine sediments--Microbiology--North Carolina--Bradley Creek, Water quality--North Carolina--Bradley Creek
- Subjects
- Bacterial pollution of water -- North Carolina -- Bradley Creek
- Water quality -- North Carolina -- Bradley Creek
- Marine sediments -- Microbiology -- North Carolina -- Bradley Creek
- Enterococcus -- Environmental aspects -- North Carolina -- Bradley Creek