Bioactive metabolites from microorganisms
- UNCW Author/Contributor (non-UNCW co-authors, if there are any, appear on document)
- Allison K. Drummond (Creator)
- Institution
- The University of North Carolina Wilmington (UNCW )
- Web Site: http://library.uncw.edu/
- Advisor
- Eve Wright
Abstract: Secondary metabolites produced by microorganisms were isolated and identified. These
metabolites are not essential for the growth of the producing culture, are usually synthesized as
closely related members of a chemical family and include compounds that act as hormones,
antibiotics and toxins. In the first chapter, actinomycetes were isolated from local marine
sediments and cultivated in order to determine bioactive metabolites of interest. Broad spectrum
antibiotic compounds produced as constitutional isomers were isolated from a strain identified as
Streptomyces malaysiensis. Additionally, a family of compounds which selectively inhibited
Mycobacterium smegamatis, a surrogate test organism used in the discovery of tuberculosis
treatments, were isolated from a strain identified as Streptomyces fimicarius. In the second
chapter, microcystin-LR, a potent liver toxin, and its derivatives were isolated from laboratory
cultures of Microcystis aeruginosa. During the isolation of these toxic metabolites, additional
unrelated peptides belonging to the microginin class of cyanopeptide metabolites were isolated
and identified. Structural determination of these five cyanopeptides revealed unique structural
features including the amino acid methionine, various sites of methylation on the modified
octanoic acid chain and the first tripeptide microginin.
Bioactive metabolites from microorganisms
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Created on 1/1/2009
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Additional Information
- Publication
- Thesis
- A Thesis Submitted to the University of North Carolina at Wilmington in Partial Fulfillment of the Requirement for the Degree of Masters of Science
- Language: English
- Date: 2009
- Keywords
- Metabolites--Analysis, Microbial metabolites--Analysis, Microcystins, Microcystis aeruginosa
- Subjects
- Microcystins
- Microcystis aeruginosa
- Metabolites -- Analysis
- Microbial metabolites -- Analysis