The Effect of Estradiol 17B on Ornithine Decarboxylase and Insulin-like Growth Factor I During Growth

UNCP Author/Contributor (non-UNCP co-authors, if there are any, appear on document)
Joseph Christopher Holt (Creator)
The University of North Carolina at Pembroke (UNCP )
Web Site:
Harold D. Mawell

Abstract: There is no doubt that estrogens like Estradiol 17B are potent simulators of the growth process by exerting their effect in many ways and through many different processes. Ornithine Decarboxylase (ODC) and Insulin-like Growth Factors I (IFG-I) are just two of the many that Estradiol 17B stimulates. The estrogenic induction of increased ODC levels is believed to occur at the messenger RNA level whereas it has been shown that the same mechanisms are responsible for the increased activity of IGF-I. An attempt was made to reveal with experimental evidence that indeed Estradiol 17B elevates ODC levels via mRNA; however, due to unforeseen complications, a denatured probe, and the crucial element of time, the means for detecting ODC were not viable. As a result, another growth index, of interest, was used to illustrate that Estradiol 17B effects the growth process in other ways as well. A non-isotopic probe, a biotinylated oligonucleotide, was used to detect the increased activity of IFG-I. However, through our research, we were able to show that biotinylated probes are not effective enough to produce conclusive results about IGF-I activity; non-isotopic probes like the particular one we used probably, perform much more efficiently when the expression of their complement is high. However, the expression of IGF-I's mRNA is minimally low and probably explains for this inconclusiveness. Other research has indicated that there is a direct relationship between IGF-I and ODC; it has be postulated that Estradiol 17B stimulates IGF-I which in turn stimulates ODC and so on with the end result being growth. With this research laying some groundwork and opening some new dors, the possibility of a role between IGF-I and ODC can be shown by establishing a temporal relationship between these two growth related factors.

Additional Information

Language: English
Date: 1991
Ornithine Decarboxylase, Insulin-like Growth Factors, Estradiol 17B, Estrogen, Biotinylation

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