Solid Phase Extraction and High Performance Liquid Chromatography Determination of Dextromehtorphan in Hair after Exposure to Cosmetic Treatment

UNCP Author/Contributor (non-UNCP co-authors, if there are any, appear on document)
Amy Avirett (Creator)
The University of North Carolina at Pembroke (UNCP )
Web Site:
Meredith Storms

Abstract: A high-performance liquid chromatography method has been developed for the determination of dextromethorphan in hair. The separation and quantitation are achieved on a phenyl column (4.6 x 150 mm) using a mobile phase of 80:20% v/v 6.25 mM sodium phosphate buffer (pH 3.0) and acetonitrile at a flow rate of 1.0 mL/min with UV detection at 226nm. The retention time for dextromethorphan is within four minutes. The method showed linearity for dextromethorphan in the 0.20-1.00 mg/mL range. The sensitivity of the method was determined to be a concentration of 0.025 mg/mL or 25 µg/mL. The inter-day RSDs ranged from 0.86 to 9.60%. A solid-phase extraction method has been developed for the extraction of dextromethorphan from hair using a Strata C18 cartridge. This method yielded an average percent recovery of 80.84% on blank, untreated hair. The SPE-HPLC was also employed to determine the effects of cosmetic treatment on the concentration of dextromethorphan in hair. Commercially available bleach (Sun-In®) and hair dye (Clairol® Natural Instincts Loving Care #75) were applied in vitro to hair strands spiked with the dextromethorphan drug. In these hair samples, the drug levels had been reduced but distinct tendencies could not be observed. Further research and investigations must be conducted in order to conclude the effects of such cosmetic treatment on the concentration of dextromethorphan in hair.

Additional Information

Honors Project
Language: English
Date: 2008
High Performance Liquid Chromatography, Liquid Chromatography, Extraction (Chemistry),

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