Degraded Samples in DNA Profiling: A literature review

UNCP Author/Contributor (non-UNCP co-authors, if there are any, appear on document)
Lee Bartalone (Creator)
Institution
The University of North Carolina at Pembroke (UNCP )
Web Site: http://www.uncp.edu/academics/library

Abstract: "This literature review covers multiple aspects of degradation in samples that may result in an altered DNA profile. The main elements that may impact a profile include but are not limited to temperature, moisture, burial depth, nucleic acid type, kits used, extraction procedure used, collection and preservation method, the presence of inhibitors, and tissue origin. All these factors may contribute to the value obtained during quantification that indicates the level of degradation in a sample otherwise known as the Degradation Index (DI). The DI of a sample has been suggested to influence the quality of a DNA profile, and in most cases, a high DI may lead to a partial/incomplete profile or even no profile at all."

Additional Information

Publication
Honors Project
Language: English
Date: 2023
Keywords
DNA degradation, DNA profiling, DNA fingerprinting, STR, PCR, NGS, SNP

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