Development of a Spectroelectrochemical Assay for Bilirubin: Preliminary Spectral and voltammetric Studies

UNCP Author/Contributor (non-UNCP co-authors, if there are any, appear on document)
Anessa Janine Lewis (Creator)
Institution
The University of North Carolina at Pembroke (UNCP )
Web Site: http://www.uncp.edu/academics/library
Advisor
Paul Flowers

Abstract: The spectral and voltammetric properties of bilirubin ditaurate conjugate in aqueous' solution were determined by employing the indicated analytical techniques. The wavelength of maximum absorbance was found to be 450nm. The molar absorptivity was determined from plots of absorbance at 450nm versus concentration to be 49,900 cm-l M-l and was found to decrease significantly over a time period of 22 days. A bilirubin oxidation wave was observed at a peak potential of 0.S8V (vs. AgCl/Ag). This wave exhibited the expected scan rate and concentration dependances at a glassy carbon working electrode. Results indicated that the oxidation of aqueous bilirubin conjugate is a simple, diffusion-controlled electrode reaction.

Additional Information

Publication
Honors Project
Chancellor's Scholars Program
Language: English
Date: 1994
Keywords
Bilirubin ditaurate conjugate, molar absorptivity, bilirubin oxidation wave, aqueous bilirubin conjugate, diffusion-controlled electron reaction

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