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Anti-tumor natural product research focused on plants found in the southern Appalachian region

WCU Author/Contributor (non-WCU co-authors, if there are any, appear on document)
Matthew John Flood II (Creator)
Institution
Western Carolina University (WCU )
Web Site: http://www.wcu.edu/404.asp
Advisor
Jason Clement

Abstract: Natural products have been an integral part of society since the beginning of recorded history. Various types of herbs, plants, and even marine organisms have been used as both topical and ingestible remedies for a variety of health concerns and problems throughout the years. Our research efforts focus on the isolation and characterization of antitumor compounds from plant extracts from western North Carolina, a surprisingly diverse and under studied region of the country. Recent work has been done on the plant species Aralia racemosa and Arnoglossum atriplicifolium. Crude extracts were initially separated using either a modified Kupchan Partitioning or standard acid/base extraction, and then further purified using various chromatographic techniques. Two compounds resulted from this work on the two species and were characterized using various methods of mass spectrometry, and advanced NMR experiments including COSY, HMBC, and HSQC. An ent-kaurane diterpenoid was isolated from Aralia racemosa, and a highly oxygenated oplopane derivative from Arnoglossum atriplicifolium. The activity of the compounds was determined through a MCF-7 assay (breast cancer), with the control group being a FS-4 assay, both carried out by Wake Forest University. This is the first report of the antitumor activity of each of these compounds.

Additional Information

Publication
Thesis
Language: English
Date: 2010
Keywords
Anti-tumor, Appalachian, Aralia racemosa, Arnoglossum atriplicifolia, Natural Products
Subjects
Materia medica, Vegetable -- Appalachian Region, Southern
Materia medica, Vegetable -- Testing
Botany, Medical -- Appalachian Region, Southern
Araliaceae -- Therapeutic use
Compositae -- Therapeutic use