Polymerase Chain Reactions on Estrogen Receptor Knockout Mice

UNCP Author/Contributor (non-UNCP co-authors, if there are any, appear on document)
Chasity Oxendine (Creator)
The University of North Carolina at Pembroke (UNCP )
Web Site: http://www.uncp.edu/academics/library
Harold D. Maxwell

Abstract: This research deals with estrogen receptor "knockout" (ERKO) mouse in which the gene for the estrogen receptor has been disrupted by gene targeting. These ERKO mice have no obvious external distinguishing features so it is necessary to determine the genotype of the offspring by polymerase chain reaction (PCR) amplification of gene gene specific sequences and visualization on ethidium bromide stained gel. I will be using a three primer technique in which all three genotypes (wildtype, herterozygote, and knockout) will result in a distinguishable pattern. This will allow for the possibility of using these combinations to determine the genotype of an unknown mouse in a single assay.

Additional Information

Language: English
Date: 1996
Genotype, Polymerase Chain Reaction, Wildtype, Heterozygote, ERKO, Mice, Estrogen Receptor Knockout, Ethidium Bromide,

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