Simultaneous Determination of 17 Ginsenosides in Rat Urine by Ultra Performance Liquid Chromatography–Mass Spectrometry with Solid-Phase Extraction

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Wei Jia, Professor and Co-Director of the UNCG Center for Research Excellence in Bioactive Food Components (Creator)
The University of North Carolina at Greensboro (UNCG )
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Abstract: A rapid analytical method for quantifying 17 ginsenosides in rat urine by ultra performance liquid chromatography (UPLC) coupled to electrospray ionization mass spectrometry (ESI-MS) is described. All analytes were extracted by solid-phase extraction optimized to obtain good recovery and quantified using digoxin as an internal standard. ESI-MS was optimized for different cone voltages at positive ionization mode to allow simultaneous analysis of all analytes in a relatively short time. Qualitative methodological considerations, including the linear range, precision, limit of quantification, limit of detection, recovery and sensitivity are also provided.

Additional Information

Analytica Chimica Acta, 594, 265-273
Language: English
Date: 2007
saponin, ginsenoside, solid-phase extraction, rat urine, ultra performance liquid chromatography–mass spectrometry

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