Determination of Daidzein in Rat Plasma by LC

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Wei Jia, Professor and Co-Director of the UNCG Center for Research Excellence in Bioactive Food Components (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/

Abstract: Daidzein (4',7-dihydroxylisoflavone), a water-insoluble isoflavone, is mainly present in leguminous plants. This study was aimed at investigating the absorption characteristics of daidzein across the intestinal membranes. Borneol has been used to enhance the intestinal absorption and bioavailability of daidzein. We chose an in vitro diffusion chamber system using isolated rat intestinal membranes as a model. A sensitive, accurate and reliable LC method was developed and validated to determine daidzein in rat plasma. A linear relationship for daidzein was found in the range of 20–800 ng mL-1. The mean absolute recoveries of daidzein determined over three concentrations were 89.8 ± 4.8, 91.3 ± 2.8 and 95.4 ± 1.7%. The coefficients of variation for inter-day and intra-day assay were found to be less than 6.1%. The plasma concentration of daidzin following oral administration was significantly increased by coadministration of borneol in rats, and a 1.5-fold enhancement of relative bioavailability of daidzein was observed as compared to daidzein administered alone.

Additional Information

Publication
Chromatographia, 68(3-4), 201-205
Language: English
Date: 2008
Keywords
column liquid chromatography, bioavailability, intestinal absorption, absorption enhancer, daidzein in rat plasma