Metabolite Profiling of Panax notoginseng Using UPLC–ESI-MS

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Wei Jia, Professor and Co-Director of the UNCG Center for Research Excellence in Bioactive Food Components (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/

Abstract: The metabolite profiling of different parts of Panax notoginseng was carried out using rapid ultra-performance liquid chromatography–electrospray ionization mass spectrometry (UPLC–ESI-MS) and multivariate statistical analysis. Principal component analysis (PCA) of the UPLC–ESI-MS data showed a clear separation of compositions among the flower buds, roots and rhizomes of P. notoginseng. The saponins accounting for such variations were identified through the corresponding loadings weights and were further verified by accurate mass, tandem mass and retention times of available standard saponins using UPLC quadrupole time-of-flight mass spectrometer (UPLC–QtofMS). Finally, the influential factors of different metabolic phenotypes of P. notoginseng was elucidated. The currently proposed UPLC–ESI-MS/MS analytical method coupled with multivariate statistical analysis can be further utilized to evaluate chemical components obtained from different parts of the plant and/or the plant of different geographical locations, thereby classifying the medicinal plant resources and potentially elucidating the mechanism of inherent phytochemical diversity.

Additional Information

Publication
Phytochemistry, 69, 2237-2244
Language: English
Date: 2008
Keywords
Panax notoginseng, metabolite profiling, saponin, ultra-performance liquid chromatography (UPLC), electrospray ionization (ESI), quadrupole time-of-flight mass spectrometer (QtofMS), multivariate statistical analysis