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The Ecdysoneless (ecd1ts) Mutation Disrupts Ecdysteroid Synthesis Autonomously in the Ring Gland of Drosophila melanogaster

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Vincent C. Henrich, Professor (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/

Abstract: Ring glands dissected from homozygous l(3)ecd1ts wandering larvae and upshifted in vitro to the restrictive temperature, 29°C, synthesize abnormally low quantities of ecdysteroid. Nevertheless, ecd1 ring glands retain the ability to respond at 29°C to an extract prepared from wild-type larval neural tissues that presumably contain prothoracicotropic hormone (PTTH), although both basal and stimulated levels of synthesis are lower than those in wild-type ring glands. Extracts prepared from ecd1 neural tissue exhibit an unusually high level of PTTH activity. Mutant ring glands downshifted in vitro to the permissive temperature after removal from larvae maintained at 29°C regain the ability to produce normal basal and stimulated ecdysteroid levels. Collectively, these experiments demonstrate that the ecd1 mutation disrupts the physiology of the ring gland at 29°C autonomously and may also interfere with PTTH release.

Additional Information

Publication
Developmental Biology
Language: English
Date: 1987
Keywords
Ecdysoneless (ecd1ts), mutation, Drosophila melanogaster