EXPLORING THE BYSTANDER EFFECT FOLLOWING VERY LOW DOSE RADIATION

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Sarah Hollis (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Bystander effects are defined as the phenomenon in which unirradiated cells respond   biologically when their neighbors are irradiated. The exact mechanisms of these   cellular responses are still not known, especially in the region considered to be very low   dose radiation (<20cGy). Many studies have shown cytokine levels are altered   following exposure to radiation and may play a significant role in bringing about   bystander responses.   A non-tumorigenic (MCF10A) and tumorigenic (A375) cell line were used to determine if   chemical factors released from irradiated MCF10A cells will bring about bystander-  induced proliferation on naïve (unirradiated) A375 or MCF10A cells using the Irradiated   Conditioned Medium (ICM) model. A transfer of conditioned medium 5 hours post-  irradiation appeared to bring about a marked bystander response in the naïve A375   cultures which were observed to be maximal between 2 and 6 cGy. This response was   not observed at the 1 hour transfer of conditioned medium. Additionally, the data   suggest that there is no temporal correlation between the proliferative bystander effects   elicited and the levels of IL-6 within the conditioned medium. The data indicate that the   MCF10A ICM IL-8 levels were statistically significant only at the 6 cGy dose at 1 hour.   Although a significant proliferative bystander effect was observed in A375 cells when   exposed to 5 hour MCF10A ICM, the data suggest that both IL-6 and IL-8 do not   contribute significantly to this proliferative response.  

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Thesis

Date: 1905

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