PI(4,5)P2 concentration at the APC side of the Immunological Synapse is Required for Effector T cell Function

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Sarah Boyle (Creator)

Michael Edidin (Creator)

David Robert Fooksman (Creator)

Saame Raza Shaikh (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Little is known about the signaling that occurs in an antigen presenting cell (APC) during contact with a T cell. Here we report the concentration of the signaling lipid, PI(4,5)P2, at the APC side of the immunological synapse. In both human and mouse cells, a PI(4,5)P2-specific fluorescent reporter, PH-GFP, detected an antigen-dependent enrichment of PI(4,5)P2 at the synapse between antigen- specific T cells and APC. When PIP(4,5)P2 was sequestered by a high concentration of PH-GFP reporter, cells were less susceptible to CTL-mediated lysis than control cells. These findings suggest a new regulatory target for modulating immune function that may be exploited for immune escape by pathogens and tumors. Originally published Journal of Immunology, Vol. 182, No. 9, May 2009

Additional Information

Publication

Other

Journal of Immunology\; 182:9 p. 5179-5182

Language: English

Date: 2023

Subjects

Cytotoxicity;T cells cytotoxic;MHC;Antigen Presentation

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