Claudin-7 modulates cell-matrix adhesion that controls cell migration, invasion and attachment of human HCC827 lung cancer cells

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Yan-Hua Chen (Creator)

DO HYUNG KIM (Creator)

QUN LU (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Claudins are a family of tight junction proteins, and serve important roles in epithelial barrier, selective ion transports and cancer metastasis. Although the exact role of claudin-7 in human lung cancer has not been completely elucidated, recent clinical studies have demonstrated that claudin-7 is associated with the survival of patients with lung cancer. Our previous studies have demonstrated that claudin-7 forms a protein complex with integrin ß1 in human lung cancer cells. The knockdown (KD) of claudin-7 by short hairpin RNA (shRNA) reduced integrin ß1 expression and increased the cell proliferative rate, whereas claudin-7 re-expression in the KD cells decreased the cell proliferation. It is unknown as to whether claudin-7 and integrin ß1 regulate cell proliferation and invasion synergistically or independently. In the present study, it was observed that ectopic expression of integrin ß1 in claudin-7 KD lung cancer cells did not reduce the cell proliferation. However, integrin ß1-transfected cells migrated more effectively in wound healing and cell invasion assays and were more adhesive in a cell attachment assay when compared with those of claudin-7 KD cells. This indicates that claudin-7 controls cell proliferation, while cell attachment and motility were regulated partially through integrin ß1. Additionally, claudin-7 overexpression in claudin-7 KD cells resulted in an improved ability to attach to the surface of cell culture plates and a higher expression of focal adhesion proteins when compared with claudin-7 non-KD control cells, which supports the role of claudin-7 in cell adhesion and motility. Taken together, these data suggest that claudin-7 regulates cell motility through integrin ß1, providing additional insight into the roles of claudins in carcinogenesis and cancer cell metastasis.

Additional Information

Publication

Other

Language: English

Date: 2023

Subjects

tight junctions\; claudin-7\; cell migration\; cell invasion\; cell attachment\; human lung cancer cells

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