Fourteen Polymorphic Microsatellite Markers For A Widespread Limestone Endemic, Carex eburnea (Cyperaceae: Carex sect. Albae)

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Matt Estep, Assistant Professor (Creator)
Nikolai M. Hay (Creator)
Institution
Appalachian State University (ASU )
Web Site: https://library.appstate.edu/

Abstract: Carex L. is a taxonomically challenging, cosmopolitan genus comprising approximately 2000 species (Reznicek, 1990), many of which possess unusually small (Nishikawa et al., 1984) but labile genomes (Lipnerová et al., 2013). This complexity presents challenges at all taxonomic levels. Carex sect. Albae (Asch. & Graebn.) Kük., like most Carex sections, has no microsatellite markers developed to address evolutionary dynamics among recently diverged species, where many taxonomic issues occur. One small but challenging group is the C. eburnea–C. mckittrickensis complex. Species boundaries between C. eburnea Boott and C. mckittrickensis P. W. Ball are unclear based on randomly amplified inter-simple sequence repeat (ISSR) markers (Gillespie, 2005) and on trnS(GCU)-trnG(UUC) and 3'trnV(UAC)-ndhC chloroplast intergenic spacer data (E. Gillespie, Marshall University, unpublished data). Additionally, morphological characters vary continuously (Ball, 1998) across the two species, making this taxon an excellent target for microsatellite marker development.

Additional Information

Publication
Emily L. Gillespie, Annabella G. Pauley, Megan L. Haffner, Nikolai M. Hay, Matt C. Estep, & Zack E. Murrell. "Fourteen Polymorphic Microsatellite Markers for a Widespread Limestone Endemic, Carex eburnea (Cyperaceae: Carex sect. Albae)," Applications in Plant Sciences, 5(8), (21 August 2017). Publisher version of record available at: https://doi.org/10.3732/apps.1700031
Language: English
Date: 2017
Keywords
Carex eburnea, Carex mckittrickensis, Carex sect. Albae, Cyperaceae, genetic diversity, limestone endemic

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