Deletion of BmoR affects the expression of genes related to thiol/disulfide balance in Bacteroides fragilis

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Felipe L.,Pauer,Heidi,Costa,Scarlathe B.,Smith,C. Jeffrey Teixeira (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Bacteroides fragilis, an opportunistic pathogen and commensal bacterium in the gut, is one the mostaerotolerant species among strict anaerobes. However, the mechanisms that control gene regulation inresponse to oxidative stress are not completely understood. In this study, we show that the MarR typeregulator, BmoR, regulates the expression of genes involved in the homeostasis of intracellular redoxstate. Transcriptome analysis showed that absence of BmoR leads to altered expression in total of 167genes. Sixteen of these genes had a 2-fold or greater change in their expression. Most of these genesare related to LPS biosynthesis and carbohydrates metabolism, but there was a signifcant increasein the expression of genes related to the redox balance inside the cell. A pyridine nucleotide-disulfdeoxidoreductase located directly upstream of bmoR was shown to be repressed by direct binding ofBmoR to the promoter region. The expression of two other genes, coding for a thiosulphate:quinoneoxidoreductase and a thioredoxin, are indirectly afected by bmoR mutation during oxygen exposure.Phenotypic assays showed that BmoR is important to maintain the thiol/disulfde balance in the cell,confrming its relevance to B. fragilis response to oxidative stress.

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Other

Language: English

Date: 2018

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