Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum
- ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)
- Yuling,Zhang,Baohong,Wen,Xinpeng,Zhang,Shulin,Wei, Liu (Creator)
- Institution
- East Carolina University (ECU )
- Web Site: http://www.ecu.edu/lib/
Abstract: A bacterial artificial chromosome (BAC) library for G. mustelinum Miers ex G. Watt (AD4)was constructed. Intact nuclei from G. mustelinum (AD4) were used to isolate high molecularweight DNA, which was partially cleaved with Hind III and cloned into pSMART BAC (HindIII) vectors. The BAC library consisted of 208,182 clones arrayed in 542 384-microtiterplates, with an average insert size of 121.72 kb ranging from 100 to 150 kb. About 2% of theclones did not contain inserts. Based on an estimated genome size of 2372 Mb for G. mustelinum, the BAC library was estimated to have a total coverage of 10.50 × genome equivalents. The high capacity library of G. mustelinum will serve as a giant gene resource formap-based cloning of quantitative trait loci or genes associated with important agronomictraits or resistance to Verticillium wilt, physical mapping and comparative genome analysis.
Additional Information
- Publication
- Other
- Language: English
- Date: 2018
| Title | Location & Link | Type of Relationship |
| Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum | http://hdl.handle.net/10342/8417 | The described resource references, cites, or otherwise points to the related resource. |