a-Klotho Expression in Mouse Tissues Following Acute Exhaustive Exercise

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Zhijian,Zheng,Lifang,Huang,Hu,Feng,Yu,Shi,Rengfei Rao (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: a-Klotho, a multifunctional protein, has been demonstrated to protect tissues frominjury via anti-oxidation and anti-inflammatory effects. The expression of a-klotho isregulated by several physiological and pathological factors, including acute inflammatorystress, oxidative stress, hypertension, and chronic renal failure. Exhaustive exercise hasbeen reported to result in tissue damage, which is induced by inflammation, oxidativestress, and energy metabolism disturbance. However, little is known about the effectsof exhaustive exercise on the expression of a-klotho in various tissues. To determine theeffects, the treadmill exhaustion test in mice was performed and the mice were sacrificedat different time points following exhaustive exercise. Our results confirmed that thefull-length (130 kDa) and shorter-form (65 kDa) a-klotho were primarily expressed inthe kidneys. Moreover, we found that, except for the kidneys and brain, other tissuesprimarily expressed the shorter-form a-klotho, including liver, which was in contrast toprevious reports. Furthermore, the shorter-form a-klotho was decreased immediatelyfollowing the acute exhaustive exercise and was then restored to the pre-exercise levelor even higher levels in the next few days. Our results indicate that a-klotho may play akey role in the body exhaustion and recovery following exhaustive exercise.

Additional Information

Publication

Other

Language: English

Date: 2019

Keywords

exhaustive exercise, a-klotho, skeletal muscle, liver, adipose tissue

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