Small RNA Sequencing Reveals Regulatory Roles of MicroRNAs in the Development of Meloidogyne incognita

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Huawei,Nichols,Robert L.,Qiu,Li,Sun,Runrun,Zhang,Baohong,Pan,Xiaoping Liu (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: MicroRNAs (miRNAs) are an extensive class of small regulatory RNAs. Knowing thespecific expression and functions of miRNAs during root-knot nematode (RKN) (Meloidogyne incognita)development could provide fundamental information about RKN development as well as a means todesign new strategies to control RKN infection, a major problem of many important crops. Employinghigh throughput deep sequencing, we identified a total of 45 conserved and novel miRNAs fromtwo developmental stages of RKN, eggs and J2 juveniles, during their infection of cotton (Gossypiumhirsutum L.). Twenty-one of the miRNAs were differentially expressed between the two stages.Compared with their expression in eggs, two miRNAs were upregulated (miR252 and miRN19),whereas 19 miRNAs were downregulated in J2 juveniles. Nine miRNAs were expressed at highlevels, with >1000 reads per mapped million (RPM) sequenced reads in both eggs and J2 juveniles(miR1, miR124, miR2-3p, miR252, miR279, miR57-5p, miR7904, miR87, and miR92). Three miRNAswere only expressed in eggs (miR4738, miRN3, and miRN5). These differentially expressed miRNAsmay control RKN development by regulating specific protein-coding genes in pathways associatedwith RKN growth and development.

Additional Information

Publication

Other

Language: English

Date: 2019

Keywords

root-knot nematode; cotton infection; small RNA; gene regulation; deep sequencing

Email this document to