Hypoxia inducible factor-1a-dependent epithelial to mesenchymal transition under hypoxic conditions in prostate cancer cells

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Mingchuan,Wang,Yong Xing,Luo,Yong,Zhao,Jiahui,Li,Qing,Zhang,Jiao,Jiang Li (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Prostate cancer is the most commonly diagnosedcancer in men and the second leading cause of cancer death.Hypoxia is an environmental stimulus that plays an importantrole in the development and cancer progression especially forsolid tumors. The key regulator under hypoxic conditions isstabilized hypoxia-inducible factor (HIF)-1a. In the presentstudy, immune-fluorescent staining, siRNAs, qRT-PC, immunoblotting, cell migration and invasion assays were carriedout to test typical epithelial to mesenchymal transition underhypoxia and the key regulators of this process in PC3, a humanprostate cancer cell line. Our data demonstrated that hypoxiainduces diverse molecular, phenotypic and functional changesin prostate cancer cells that are consistent with EMT. Wealso showed that a cell signal factor such as HIF-1a, whichmight be stabilized under hypoxic environment, is involved inEMT and cancer cell invasive potency. The induced hypoxiacould be blocked by HIF-1a gene silencing and reoxygenationof EMT in prostate cancer cells, hypoxia partially reversedaccompanied by a process of mesenchymal-epithelial revertingtransition (MErT). EMT might be induced by activation ofHIF-1a-dependent cell signaling in hypoxic prostate cancercells.

Additional Information

Publication

Other

Language: English

Date: 2016

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