De Novo Transcriptome Assembly and Comparative Analysis Elucidate Complicated Mechanism Regulating Astragalus chrysochlorus Response to Selenium Stimuli

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)
Özgür,Turgut-Kara1,Neslihan,Arı,Şule,Zhang,Bao Çakır (Creator)
Institution
East Carolina University (ECU )
Web Site: http://www.ecu.edu/lib/

Abstract: Astragalus species are medicinal plants that are used in the world for years. Some Astragalus species are known for selenium accumulation and tolerance and one of them isAstragalus chrysochlorus, a secondary selenium accumulator. In this study, we employedIllumina deep sequencing technology for the first time to de novo assemble A. chrysochlorus transcriptome and identify the differentially expressed genes after selenate treatment. Totally, 59,656 unigenes were annotated with different databases and 53,960unigenes were detected in NR database. Transcriptome in A. chrysochlorus is closer toGlycine max than other plant species with 43,1 percentage of similarity. Annotated unigenes were also used for gene ontology enrichment and pathway enrichment analysis.The most significant genes and pathways were ABC transporters, plant pathogen interaction, biosynthesis of secondary metabolites and carbohydrate metabolism. Our results willhelp to enlighten the selenium accumulation and tolerance mechanisms, respectively inplants

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Other
Language: English
Date: 2015

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De Novo Transcriptome Assembly and Comparative Analysis Elucidate Complicated Mechanism Regulating Astragalus chrysochlorus Response to Selenium Stimulihttp://hdl.handle.net/10342/8135The described resource references, cites, or otherwise points to the related resource.