High-Performance Liquid Chromatographic Determination of Metformin in Human Serum Using Solid-Phase Extraction

UNCP Author/Contributor (non-UNCP co-authors, if there are any, appear on document)
Danielle Chavis (Creator)
The University of North Carolina at Pembroke (UNCP )
Web Site: http://www.uncp.edu/academics/library
Meredith Storms

Abstract: A high-performance liquid chromatography method has been developed for the determination of metformin in human serum. The separation and quantitation are achieved on a 150-cm Luna C18 column using a mobile phase of 60:40% v/v 10mM sodium phosphate buffer containing 10 mM SDS (pH 5.0) and acetonitrile at a flow rate of 0.5mL/min with detection of metformin at 226 nm. Recoveries found from a solid-phase extraction (SPE) method for metformin in human serum ranged from 92.4-100.5%. The highest recovery was found using a Phenyl SPE cartridge eluted with 0.75mL ACN while other cartridges yielded lower recoveries.

Additional Information

Honors Project
Language: English
Date: 2007
Liquid Chromatography, Metformin, Human Serum, Sodium Phosphate Buffer, Acetonitrile, Solid-phase Extraction, Pheynl SPE cartridge,

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