Development of a Method for the Determination of Bisphenol A in Vervet Monkey Hair using LC-MS/MS and LC-UV/Vis

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Samuel Joseph Lindsay (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: No methods for estimating levels of anthropogenic disturbance on non-human primate (NHP) populations were identified within the literature. Such a method would allow for the assessment of human impact on NHP populations. Levels of bisphenol A (BPA) in hair samples was the proposed metric for such a method. Human hair samples were used to validate a procedure found in the literature.1 A methanolic extraction was performed on human hair and the resulting samples were analyzed using liquid chromatography tandem mass spectrometry (LC-MS/MS). The two extracted samples contained 110.1 and 37.1 pg BPA mg-1 hair. Extracted , spiked samples yielded 913.9 and 885.7 pg BPA mg-1 hair. Control samples of standard yielded 200 times lower than the expected concentration. A control alkaline extraction was attempted but not found to be suitable. Standards over the expected concertation range of prepared extractions were run on a liquid chromatography - ultraviolet visible spectrophotometry (LC-UV/Vis) system were used to generate a standard curve with an R2 value of 0.9993.

Additional Information

Publication

Thesis

Language: English

Date: 2019

Keywords

pollution, environment, plastic

Subjects

Email this document to