Development of a Method for the Determination of Bisphenol A in Vervet Monkey Hair using LC-MS/MS and LC-UV/Vis

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)
Samuel Joseph Lindsay (Creator)
Institution
East Carolina University (ECU )
Web Site: http://www.ecu.edu/lib/

Abstract: No methods for estimating levels of anthropogenic disturbance on non-human primate (NHP) populations were identified within the literature. Such a method would allow for the assessment of human impact on NHP populations. Levels of bisphenol A (BPA) in hair samples was the proposed metric for such a method. Human hair samples were used to validate a procedure found in the literature.1 A methanolic extraction was performed on human hair and the resulting samples were analyzed using liquid chromatography tandem mass spectrometry (LC-MS/MS). The two extracted samples contained 110.1 and 37.1 pg BPA mg-1 hair. Extracted , spiked samples yielded 913.9 and 885.7 pg BPA mg-1 hair. Control samples of standard yielded 200 times lower than the expected concentration. A control alkaline extraction was attempted but not found to be suitable. Standards over the expected concertation range of prepared extractions were run on a liquid chromatography - ultraviolet visible spectrophotometry (LC-UV/Vis) system were used to generate a standard curve with an R2 value of 0.9993.

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Publication
Thesis
Language: English
Date: 2019
Keywords
pollution, environment, plastic
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Development of a Method for the Determination of Bisphenol A in Vervet Monkey Hair using LC-MS/MS and LC-UV/Vishttp://hdl.handle.net/10342/7383The described resource references, cites, or otherwise points to the related resource.