Modulation of experimental autoimmune myocarditis with cytokine-myosin fusion proteins and novel cardiac peptides

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)
Shaun Patrick Reece (Creator)
East Carolina University (ECU )
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Abstract: Infectious agents that induce cross-reactive myocarditic T cell responses may cause autoimmune inflammation of the heart , dilated cardiomyopathy (DCM) , and heart failure. The purpose of this study was to evaluate the efficacy of GMCSF-Myosin fusion proteins as tolerogenic vaccines capable of inhibiting experimental autoimmune myocarditis (EAM). The fusion proteins were comprised of an N-terminal granulocyte macrophage-colony stimulating factor (GM-CSF) domain and a C-terminal cardiac myosin antigen domain. The antigenic domains Myosin 1052-1076 and Myosin 614-643 were chosen based on epitopes known to cause EAM in Lewis rats and BALB/c mice , respectively. Bioactivity of the cytokine domains for GMCSF-Myo1052 and GMCSF-Myo614 were confirmed with bone marrow proliferation assays. Enhanced antigen presentation was confirmed for GMCSF-Myo1052. Both fusion proteins were found to inhibit disease progression when given as a pretreatment before EAM induction. When administered after disease induction , GMCSF-Myo614 reduced the incidence of EAM. To investigate the mechanisms of these tolerogenic vaccines , a model of EAM was developed in C57BL/6 mice by evaluating a peptide (myosin 718-736) derived from the murine cardiac alpha myosin heavy chain and theorized to bind to H2-I-Ab. EAM was successfully induced in Interferon gamma receptor knockout (Ifgr1-/- ) mice on the C57BL/6 background. Defining the mechanisms by which Interferon-gamma (IFN-[gamma]) inhibits EAM on the C57BL/6 background is beyond the scope of this project , but it is hypothesized that T cell apoptosis or induction of T regulatory cells is involved. In conclusion , this study demonstrated the feasibility of using GMCSF-Myosin fusion proteins to inhibit EAM , and provides a new model of EAM on C57BL/6 background to explore the mechanisms by which GMCSF-Myosin fusion proteins mediate tolerance.

Additional Information

Language: English
Date: 2016
Experimental autoimmune myocarditis, Fusion proteins, Cardiac peptides

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