Constituents, biological activities and quality control parameters of the crude extract and essential oil from Arracacia tolucensis var. multifida

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Mario Figueroa Saldivar, Adjunct Faculty (Creator)
The University of North Carolina at Greensboro (UNCG )
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Abstract: Bioassay guided fractionation of an antimycobacterial extract of Arracacia tolucensis var. multifida (Umbelliferae) led to the isolation of isoimperatorin (1), osthol (2), suberosin (3), 8-methoxypsoralen (8-MOP) (4), herniarin (5), scoparone (6), umbelliferone (7), dihydroxypeucedanin (8), 5-methoxypsoralen (5-MOP) (9), isoscopoletin (10) and scopoletin (11). The isolates were tested against Mycobacterium tuberculosis and only 1–4 showed significant activity with MIC values of 64, 32, 16 and 128 µg/mL, respectively. The essential oil showed moderate in vitro antibacterial activity against representative Gram-positive and Gram-negative bacteria. The volatile oil of Arracacia tolucensis var. multifida was analyzed by GC–MS and found to be composed mainly by 2 and 3. The essential oil (IC50 = 116.4 ± 23.2 µg/mL) and the extract (IC50 = 1153.1 ± 53.2 µg/mL) of the plant provoked concentration dependent inhibition of the tone and amplitude of the guinea-pig ileum spontaneous contractions; the latter activity was related with the high coumarin content of this species. A suitable (novel and rapid) HPLC method to quantify the major active coumarins of the plant was developed. The method provides also a reproducible fingerprint useful for identity tests of this plant.

Additional Information

Journal of Ethnopharmacology, 113 (1), pp. 125-131
Language: English
Date: 2007
Arracacia tolucensis var. multifida, Spasmolytic activity, Antimicrobial activity, Coumarins, Antimycobacterial, Umbelliferae

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