Calmodulin inhibitors from the fungus Emericella sp.
- UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
- Mario Figueroa Saldivar, Adjunct Faculty (Creator)
- Institution
- The University of North Carolina at Greensboro (UNCG )
- Web Site: http://library.uncg.edu/
Abstract: Two new xanthones identified as 15-chlorotajixanthone hydrate (1) and 14-methoxytajixanthone (2) were isolated from an Emericella sp. strain 25379 along with shamixanthone (3) and tajixanthone hydrate (4). The stereostructures of 1 and 2 were elucidated by spectroscopic and molecular modeling methods. The absolute configuration at the stereogenic centers of 1 was established according to CD measurements. In the case of 2, however, the absolute configuration at C-20 and C-25 was designated as S and R, respectively, by Mosher ester methodology. Thereafter, the configuration at C-14 and C-15 of 2 was established as S and S, respectively by comparing the optical rotation and 1H–1H coupling constant experimental values with those obtained through molecular modeling calculations at DFT B3LYP/DGDZVP level of theory for diasteroisomers 2a–2d. The activation of the calmodulin-sensitive cAMP phosphodiesterase (PDE1) was inhibited in the presence of 1–4 in a concentration-dependent manner. The effect of compounds 2 (IC50 = 5.54 µM) and 4 (IC50 = 5.62 µM) was comparable with that of chlorpromazine (CPZ; IC50 = 7.26 µM), a well known CaM inhibitor used as a positive control. The inhibition mechanism of both compounds was competitive with respect to CaM according to a kinetic study. A docking analysis with 2 and 4 using the AutoDock 4.0 program revealed that they interacted with CaM in the same pocket as trifluoropiperazine (TFP).
Calmodulin inhibitors from the fungus Emericella sp.
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Additional Information
- Publication
- Bioorganic and Medicinal Chemistry, 17 (6), pp. 2167-2174
- Language: English
- Date: 2009
- Keywords
- Emericella, 15-Chlorotajixanthone hydrate, 14-Methoxytajixanthone, Shamixanthone, Tajixanthone hydrate, Calmodulin, Docking