Effects of respiratory inhibitors on intermediate stages in the development of the mi-1 phenotype in neurospora
- UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
- Edwin Frederick Slott (Creator)
- Institution
- The University of North Carolina at Greensboro (UNCG )
- Web Site: http://library.uncg.edu/
- Advisor
- William Bates
Abstract: Previous studies have demonstrated that the phenotypic character of the cytoplasmic mutant mi-1 can be transmitted to recipient wild type cell by micro-injection of isolated mi-1 mitochondria. During serial transfers from the injected cell, a phenotypic lag occurs before the mi-1 phenotype appears. The present study is an examination of the developing transfers. Polarographic studies were conducted with intact cells and isolated mitochondria of critical transfers in the presence of the respiratory inhibitors cyanide and salicyl hydroxamic acid (SHAM). SHAM inhibits cyanide-resistant respiration in higher plants and Neurospora. Without inhibitors, the respiration rates of early transfers were equal to wild type rates. A gradual increase occurred, after which transfers remained above the wild type level, thus displaying a stable non-reverting characteristic. For whole cells and isolated mitochondria of wild type and early transfers, inhibition of respiration by cyanide was complete. At Transfer six there was an abrupt resistance to cyanide as is characteristic of mi-1. In whole cells and isolated mitochondria, SHAM-sensitive respiration develops between Transfers five and eight. Although significantly above wild type levels, this SHAM-sensitivity does not reach the level characteristic of mi-1.
Effects of respiratory inhibitors on intermediate stages in the development of the mi-1 phenotype in neurospora
PDF (Portable Document Format)
1061 KB
Created on 1/1/1975
Views: 125
Additional Information
- Publication
- Thesis
- Language: English
- Date: 1975