An autoradiographic analysis of mitotic activity in imaginal wing discs of drosophila virilis in vitro

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Linda-Margaret Hunt (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site:
Edward McCrady

Abstract: One of the most extensively studied phenomena in developmental biology today is the transition of determined cells into differentiated cells. One of the techniques most often used in this area is tissue culture. Although this method of analysis allows the direct observation of cellular and tissue interactions, the results must be constantly interpreted in terms of their relation to in situ phenomena. In the present study imaginal wing discs were excised from late third instar Drosophila virilis and cultured in vitro in Schneider's medium for zero to seven days. At the end of the culture period, the discs were labeled with tritiated thymidine for two and one half hours and sectioned for autoradiography. The developed autoradiographs were examined under light microscopy for evidence of uptake of label, which is directly correlated with the mitotic activity of the labeled cells.

Additional Information

Language: English
Date: 1971
Drosophila $x Genetics
Imaginal disks

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