Influence of cell loss during in vitro culture of drosophila wing discs

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
L. Cheryl Stimpson (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site:
Edward Crady

Abstract: Tissue culture is an effective method for study of the developmental capacity of embryonic tissue. The imaginal discs of Drosophila larvae are determined as to the structure in the adult to which they will give rise, but these discs remain undifferentiated throughout larval life. Hadorn (1966) found that by interrupting the development of one of these discs between its determination and differentiation, he could modify the amount of determination and the ability of the disc to differentiate at any given time. His experiments were carried out in vivo; that is, a determined blastema was taken from a larva and injected into the abdomen of an adult where it was allowed to proliferate. After any length of time, the tissue could be recovered and transplanted into a prepupal larva. After emergence of the adult the amount of differentiation could be determined by direct observation. Hadorn reported the occurrence of transdetermination, a phenomenon in which a determined imaginal disc differentiates into an unrelated structure (Hadorn, 1966). Futhermore, he and his students concluded that the amount of transdetermination is related to proliferation; that is, the longer the disc is allowed to grow in the adult, the more transdetermination will take place (Tobler, 1966; Garica-Bellido, 1966).

Additional Information

Honors Project
Language: English
Date: 1969

Email this document to