Optimization of linker system of potential melanoma skin cancer probe

WCU Author/Contributor (non-WCU co-authors, if there are any, appear on document)
Sarah Elizabeth Lee Ballard (Creator)
Institution
Western Carolina University (WCU )
Web Site: http://library.wcu.edu/
Advisor
Brian Dinkelmeyer

Abstract: The aim of our proposed research is to develop clinical tools for detecting cancer cells and to further expand methods for the early detection of cancer. The focus of this research is to optimize the synthesis of a probe for detecting melanocyte stimulating hormone(MSH) G-proteins that are present in melanocyte cancer cells. An europium complex was chosen since it is an excellent luminescent tag with a narrow emission band and has a long luminescent lifetime. MSH-4 peptide was selected as the analyte since it binds specifically with MSH G proteins in melanoma cells. The melanocyte stimulating hormone (MSH-4) and its analogs were synthesized via a solid phase peptide synthesis method using F-moc protocol. The lanthanide tag was synthesized by chelating 5-amine-1,10- phenanthroline to Eu(TTA)3(H2O) complex. A tetraethylene linker was successfully synthesized and attached to the phenanthroline ligand. This was done by oxidizing the monobenzyl tetraethylene glycol to a carboxylic acid and adding it to the 5-amino-1,10-phenathroline amine using a standard peptide coupling procedure. The linker synthesis was found to be reproducible. The chelation of the ligand to the europium TTA complex was also completed. All compounds synthesized were characterized using the following instrumentation: GC-MS, UV-Vis, HPLC, FT-IR, NMR and fluorescence spectroscopy.

Additional Information

Publication
Thesis
Language: English
Date: 2019
Keywords
Europium tag, linker, melanoma, peptide, probe

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