Bioactive Withanolides from Withania obtusifolia

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Tamam M. El-Elimat (Creator)
Mario Figueroa Saldivar, Adjunct Faculty (Creator)
Nicholas Oberlies, Patricia A. Sullivan Distinguished Professor of Chemistry (Creator)
The University of North Carolina at Greensboro (UNCG )
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Abstract: Seven withanolides were isolated from the leaves of Withania obtusifolia. Of these, one was new [obtusifonolide (1)], five were new to the species [sitoindoside IX (2), 6a-chloro-5ß-hydroxy withaferin A (3), isowithanone (4), 2,3-dihydro-3-ethoxywithaferin A (5), and daturataturin A (6)], and one was reported previously from W. obtusifolia [withaferin A (7)]. The structures were elucidated using a set of spectroscopic and spectrometric techniques. Compounds (1–7) were evaluated for cytotoxicity against a human cancer cell panel and for antimicrobial activity in an array of bacteria and fungi. Compound 7 showed cytotoxic activity against the MDA-MB-435 (human melanoma) and SW-620 (human colon cancer) cell lines with IC50 values of 1.7 and 0.3 µM, respectively. The in vitro activity of 7 on 17ß-hydroxysteroid dehydrogenase and 5a-reductase was also investigated.

Additional Information

Phytochemistry Letters, 9, 96-101. doi:10.1016/j.phytol.2014.05.002
Language: English
Date: 2014
Withania obtusifolia, Cytotoxicity, Withanolides, Withaferin A, 17ß-Hydroxysteroid dehydrogenase, 5a-Reductase

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