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Gold nanoparticle enhanced capillary electrophoresis separations for Alzheimer's disease biomarker detection.

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Dickson M. Wambua (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Advisor
Brent Dawson

Abstract: Alzheimer's disease (AD) is a progressive, neurodegenerative disease characterized by memory and cognitive loss, the formation of senile plaques containing amyloid-beta (Aß) peptides, degeneration of the cholinergic neurons and the development of neurofibrillary tangles in the brains of the affected people.1 It is estimate that 5.1 million people in the United States are suffering from AD. The total annual care cost associated with AD is more than $ 148 billion and is constantly increasing3. Presently, there is no specific clinical test to diagnose AD, however, amyloid beta peptides and tau proteins have been shown to be reliable biomarkers that can be used for AD diagnosis and tracking disease progression.5 It is important therefore that proper methods and techniques be devised for the identification, quantification and analysis of AD biomarkers to enable the reliable clinical diagnosis of the disease. In this study, modified gold nanoparticles were used as a pseudo stationary phase in a capillary electrochromatography technique. An asymmetric disulfide synthesized from mercaptoundecanoic acid and dodecanethiol was chemisorbed onto 10 nm gold nanoparticles to form the pseudo stationary phase. With the help of the gold nanoparticle pseudo stationary phase, the separation of two Alzheimer’s disease biomarkers namely; amyloid beta 40 and amyloid beta 42 was accomplished. This study demonstrates that the presence of modified gold nanoparticles in capillary electrochromatography can greatly influence the separation of amyloid beta peptides.

Additional Information

Publication
Thesis
Language: English
Date: 2009
Keywords
Alzheimer's disease, Biomarker, Capillary electrochromatography, Gold nanoparticles
Subjects
Alzheimer's Disease $x Research.
Nanotechnology $x Medicine.
Biochemical markers.