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Comparison and Analysis of ADH Isozymes from a Population of Chrysoma pauciflosculosa

UNCP Author/Contributor (non-UNCP co-authors, if there are any, appear on document)
Andrea M. Winter (Creator)
Institution
The University of North Carolina at Pembroke (UNCP )
Web Site: http://www.uncp.edu/academics/library
Advisor
Bonnie Kelley

Abstract: Chrysoma pauciflosculosa is a rare evergreen shrub that is located on a sandy ridge. There are two populations of Chrysoma located in Pembroke, North Carolina. No published information on isozyme studies or population genetics exists for this species. Isozymes are variable forms of an enzyme. Two enzymes, ADH and MDH, were chosen for the comparison. Two prior students at UNCP developed an initial protocol for studying isozymes in this species. There data was used for storage and grinding. The leaves were collected from one site near Purnell Swett High School in Pembroke, North Carolina. The leaves were homogenized, electrophoresed on acrylamide gels and stained for the particular enzyme being studied. It was determined that ADH was active in this population of Chrysoma.

Additional Information

Publication
Honors Project
Language: English
Date: 2004
Keywords
Isozymes, Isoenzymes, Shrubs, Chrysoma pauciflosculos, ADH Isozymes