Moringa Oleifera Phytochemical Composition And The Influence Of Environmental Growing Conditions

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Tanner Sciara (Creator)
Appalachian State University (ASU )
Web Site:
Jennifer Cecile

Abstract: Moringa oleifera is an important plant as it contains a large amount of necessary nutrients for humans including protein, vitamins, and minerals. It also could useful for other purposes such as biofuel, animal fodder, and as a water clarifier. In this work a M. oleifera ethanolic extract reconstituted in borate buffer was analyzed using absorbance, fluorescence, and capillary electrophoresis for the presumptive and quantitative measurement of the phytochemical isoquercetin. The absorbance and fluorescence data presumptively indicated that isoquercetin was present in the extract along with other phytochemicals. Capillary electrophoresis electropherogram data with the neutral marker mesityl oxide indicated that the species of interest on the electropherogram were anionic. A standard addition of isoquercetin using capillary electrophoresis separation resulted in a calculated extract concentration of 312 ± 42 µM isoquercetin. A repeat standard addition is necessary to increase precision of the isoquercetin concentration. Another aspect of this project was creating a UV supplemented, hydroponic growing model for M. oleifera in order to stimulate more phytochemical production. Prototype results were promising, however, the completed model grew plants with an abnormal morphology. Further testing is needed to determine the cause before plants grown with the model can be analyzed using the aforementioned techniques.

Additional Information

Honors Project
Sciara, T. (2018). "Moringa Oleifera Phytochemical Composition And The Influence Of Environmental Growing Conditions." Unpublished Honors Thesis. Appalachian State University, Boone, NC.
Language: English
Date: 2018
Moringa oleifera, Capillary electrophoresis, Isoquercetin, Ethanolic extract, Hydroponic

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