Synthesis and characterization of time-resolved fluorescence probes for the potential detection and improved study of melanoma cancer
- WCU Author/Contributor (non-WCU co-authors, if there are any, appear on document)
- Seth Alexander Sedberry (Creator)
- Institution
- Western Carolina University (WCU )
- Web Site: http://library.wcu.edu/
- Advisor
- Brian Dinkelmeyer
Abstract: A multi-part fluorescent probe was designed for the development of new clinical tools for the detection and treatment of melanoma skin cancer. It has been proposed that the addition of a fluorescent tag to a melanocyte stimulating hormone would create a luminescent probe with potential for the detection and study of melanoma skin cancer. The proposed design involves attaching a luminescent lanthanide probe to an MSH (4) peptide substrate using a flexible polyethylene glycol linker. The individual portions of the proposed molecule (luminescent probe, PEGO linker, and MSH (4) peptide) have been synthesized and characterized using a combination of FTIR, NMR, GCMS, HPLC, UV-Vis, and Fluorescent spectroscopy. Luminescent probe synthesis involved the nitration and subsequent reduction of 1,10-phenanthroline followed by complexation with Eu(TTA)3. Synthesis of the PEGO linker was accomplished by the reaction of tetraethylene glycol with tert-butyl bromoacetate to form a diacid through a t-butyl protected intermediate. MSH (4) peptide synthesis proceeded on solid phase following a f-moc protocol and using a Mars6 microwave synthesizer. Future work will focus on the construction of the final molecule using the components obtained though this thesis research.
Synthesis and characterization of time-resolved fluorescence probes for the potential detection and improved study of melanoma cancer
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Created on 8/1/2016
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Additional Information
- Publication
- Thesis
- Language: English
- Date: 2016
- Subjects
- Fluorescent probes
- Luminescent probes
- Fluorescence spectroscopy
- Melanoma -- Early detection
- Imaging systems in biology