Metabolomics approach to assessing plasma 13- and 9-hydroxy-octadecadienoic acid and linoleic acid metabolite responses to 75-km cycling
- ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
- Dr.. David Nieman, Director (Creator)
- Andrew Shanely Ph.D, Associate Professor (Creator)
- Institution
- Appalachian State University (ASU )
- Web Site: https://library.appstate.edu/
Abstract: Bioactive oxidized linoleic acid metabolites (OXLAMs) include 13- and 9-hydroxy-octadecadienoic acid (13-HODE + 9-HODE) and have been linked to oxidative stress, in?ammation, and numerous pathological and physiological states. The purpose of this study was to measure changes in plasma 13-HODE + 9-HODE following a 75-km cycling bout and identify potential linkages to linoleate metabolism and established biomarkers of oxidative stress (F2-isoprostanes) and in?ammation (cytokines) using a metabolomics approach. Trained male cyclists (N ?? 19, age 38.0 + 1.6 yr, wattsmax 304 +10.5) engaged in a 75-km cycling time trial on their own bicycles using electromagnetically braked cycling ergometers (2.71 + 0.07 h). Blood samples were collected preexer-cise, immediately post-, 1.5 h post-, and 21 h postexercise, and analyzed for plasma cytokines (IL-6, IL-8, IL-10, tumor necrosis factor-??, monocyte chemoattractant protein-1, granulocyte colony-stimulating factor), F2-isoprostanes, and shifts in metabolites using global metabolomics procedures with gas chromatography mass spec-trometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS). 13-HODE +9-HODE increased 3.1-fold and 1.7-fold immediately post- and 1.5 h postexercise (both P <0.001) and returned to preexercise levels by 21-h postexercise. Post-75-km cy-cling plasma levels of 13-HODE ?? 9-HODE were not signi?cantly correlated with increases in plasma cytokines but were positively correlated with postexercise F2-isoprostanes (r =0.75, P < 0.001), linoleate (r = 0.54, P = 0.016), arachidate (r = 0.77, P < 0.001), 12,13-dihydroxy-9Z-octadecenoate (12,13-DiHOME) (r = 0.60, P = 0.006), dihomo-linolenate (r =0.57, P = 0.011), and adrenate (r = 0.56, P = 0.013). These ?ndings indicate that prolonged and intensive exercise caused a transient, 3.1-fold increase in the stable linoleic acid oxidation product 13-HODE + 9-HODE and was related to increases in F2-isoprostanes, linoleate, and fatty acids in the linoleate conver-sion pathway. These data support the use of 13-HODE + 9-HODE as an oxidative stress biomarker in acute exercise investigations
Metabolomics approach to assessing plasma 13- and 9-hydroxy-octadecadienoic acid and linoleic acid metabolite responses to 75-km cycling
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Additional Information
- Publication
- David C. Nieman, R. Andrew Shanely, Beibei Luo, Mary Pat Meaney, Dustin A. Dew and Kirk L. Pappan (2014) "Metabolomics approach to assessing plasma 13- and 9-hydroxy-octadecadienoic acid and linoleic acid metabolite responses to 75-km cycling" American Journal of Physiology-Regulatory, integrative and comparative Physiology Version of Record Available @ (doi:10.1152/ajpregu.00092.2014.)
- Language: English
- Date: 2014
- Keywords
- oxidative stress, inflammation, linoleate, metabolites