Differences in transcriptional patterns of extracellular matrix, inflammatory, and myogenic regulatory genes
- ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
- Kevin Zwetsloot Ph.D, Associate Professor (Creator)
- Institution
- Appalachian State University (ASU )
- Web Site: https://library.appstate.edu/
Abstract: Aged skeletal muscle displays increased fibrosis and impaired regeneration. While it is not well characterized how skeletal muscle fibroblasts contribute to these phenomena, transforming growth factor- b1 (TGF-b1) and Delta/Notch signaling have been implicated to influence muscle regeneration. In this study, a unique combination of aging phenotypes is identified in differentiating fibroblasts (myofibro- blasts), proliferating fibroblasts, and muscle precursor cells (MPCs) that characterize an impaired regenerative potential observed in aged skeletal muscle. Using a novel dual-isolation technique, that isolates fibro- blasts and MPCs from the same rat skeletal muscle sample, and cell culture conditions of 5 % O2 and 5 % CO2, we report for the first time that myofibroblasts from 32-mo-old skeletal muscle, compared to 3-mo- old, display increased levels of mRNA for the essential extracellular matrix (ECM) genes, collagen 4a1 (83 % increase), collagen 4a2 (98 % increase), and laminin 2
Differences in transcriptional patterns of extracellular matrix, inflammatory, and myogenic regulatory genes
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Additional Information
- Publication
- Kevin A. Zwetsloot, Anders Nedergaard, Leigh T. Gilpin, Thomas E. Childs, Frank W. Booth(2012) "Differences in transcriptional patterns of extracellular matrix, inflammatory, and myogenic regulatory genes in myofibroblasts, fibroblasts, and muscle precursor cells isolated from old male rat skeletal muscle using a novel cell isolation procedure" Biogerontology 13:383–398 Version of Record available @ (DOI 10.1007/s10522-012-9382-7)
- Language: English
- Date: 2012
- Keywords
- , extracellular-matrix, inflammatory, myogenic-regulatory, myofibroblasts, fibroblasts, muscle-precursor-cells, rat-skeletal-muscle,