Methanogenesis In Mclean Bog, An Acidic Peat Bog In Upstate New York: Stimulation By H2/CO2 In The Presence Of Rifampicin, Or By Low Concentrations Of Acetate

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Suzanna Brauer Ph.D., Associate Professor (Creator)
Institution
Appalachian State University (ASU )
Web Site: https://library.appstate.edu/

Abstract: Acidic peat bog soils produce CH4 and although molecular biological studies have demonstrated the presence of diverse methanogenic populations in them, few studies have sustained methanogenesis by adding the CH4 precursors H2/CO2 or acetate, and few indigenous methanogens have been cultured.McLeanBog is a small(ca. 70 m across), acidic (pH 3.4–4.3) Sphagnum-dominated bog in upstate New York. Although addition of H2/CO2 or 10m Macetate stimulated methanogenesis in soils from a nearby circumneutral pHfen, neither of these substrates led to sustained methanogenesis in McLean Bog soil slurries. After a brief period of stimulation by H2/CO2, methanogenesis in McLean Bog soil declined, which could be attributed to buildup of large amounts of acetic acid produced from the H2/CO2 by acetogens. Addition of the antibiotic rifampicin inhibited acetogenesis (carried out by Bacteria) and allowed methanogenesis (carried out by Archaea) to continue. Using rifampicin, we were able to study effects of temperature, pH, and salts on methanogenesis from H2/CO2 in McLean Bog soil samples.The enriched H2/CO2-utilizing methanogens showed an optimum for activity near pH 5, and a temperature optimum near 35?C.Methanogenesis was not stimulated by addition of 10 mM acetate,but it was stimulated by 1 mM acetate, and multiple additions were consumed at increasing rates and nearly stoichiometrically converted to CH4. In conclusion, we have found that both hydrogentrophic and aceticlastic methanogens are present in McLeanBog soils, and that methanogenic activity can be stimulated usingH2/CO2 in the presence of rifampicin, or using low concentrations of acetate.

Additional Information

Publication
Bräuer Suzanna L, Yavitt JB, and Zinder SH. (2004). "Methanogenesis In Mclean Bog, An Acidic Peat Bog In Upstate New York: Stimulation By H2/CO2 In The Presence Of Rifampicin, Or By Low Concentrations Of Acetate." Geomicrobiology Journal, 21(7):433-443. [ISSN: 0149-0451] [DOI 10.1080/01490450490505400] Version Of Record Available At www.tandfonline.com
Language: English
Date: 2004
Keywords
acidic peat bog, rifampicin, acetate, H2/CO2, acetogens

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