Flavonolignans from Aspergillus iizukae, a Fungal Endophyte of Milk Thistle (Silybum marianum)
- UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
- Nadja B. Cech, Patricia A. Sullivan Distinguished Professor of Chemistry (Creator)
- Stanley H. Faeth, Professor Emeritus (Creator)
- Tyler Graf, Research Scientist (Creator)
- Nicholas Oberlies, Patricia A. Sullivan Distinguished Professor of Chemistry (Creator)
- Huzefa A. Raja, Research Scientist (Creator)
- Institution
- The University of North Carolina at Greensboro (UNCG )
- Web Site: http://library.uncg.edu/
Abstract: Silybin A (1), silybin B (2), and isosilybin A (3), three of the seven flavonolignans that constitute silymarin, an extract of the fruits of milk thistle (Silybum marianum), were detected for the first time from a fungal endophyte, Aspergillus iizukae, isolated from the surface-sterilized leaves of S. marianum. The flavonolignans were identified using a UPLC-PDA-HRMS-MS/MS method by matching retention times, HRMS, and MS/MS data with authentic reference compounds. Attenuation of flavonolignan production was observed following successive subculturing of the original flavonolignan-producing culture, as is often the case with endophytes that produce plant-based secondary metabolites. However, production of 1 and 2 resumed when attenuated spores were harvested from cultures grown on a medium to which autoclaved leaves of S. marianum were added. The cycle of attenuation followed by resumed biosynthesis of these flavonolignans was replicated in triplicate.
Flavonolignans from Aspergillus iizukae, a Fungal Endophyte of Milk Thistle (Silybum marianum)
PDF (Portable Document Format)
415 KB
Created on 9/28/2015
Views: 2208
Additional Information
- Publication
- Journal of Natural Products
- Language: English
- Date: 2014
- Keywords
- flavonolignan, Silybum marianum, milk thistle