Imaging and Organelle Distribution of Fluorescent InGaP/ZnS Nanoparticles in Glial Cells
- UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
- Marinella Sandros, Assistant Professor (Creator)
- Institution
- The University of North Carolina at Greensboro (UNCG )
- Web Site: http://library.uncg.edu/
Abstract: Aim: To assess the effects of oleic acid treatment on subcellular distribution of indium gallium phosphide–zinc sulfide (InGaP/ZnS) nanoparticles in microglia and astrocytes. Materials & methods: The extent of colocalization between the nanoparticles and organelles was assessed by confocal microscopy, spectrofluorometry and cell sorting. Results: Cell treatment with a common fatty acid (oleic acid) within the range of physiological concentrations markedly enhanced the InGaP/ZnS uptake by microglia and afforded their colocalization within lipid droplets/lysosomes but not with mitochondria. Conclusion: These results suggest that the availability of mono-unsaturated fatty acids, such as oleic acid, in different cells could significantly alter nanoparticle uptake and localization, which can in turn affect the functions of cells and tissues coexposed to nanoparticles.
Imaging and Organelle Distribution of Fluorescent InGaP/ZnS Nanoparticles in Glial Cells
PDF (Portable Document Format)
103 KB
Created on 12/16/2014
Views: 2053
Additional Information
- Publication
- Nanomedicine, 4(7), 747-761
- Language: English
- Date: 2009
- Keywords
- cell organelles, imaging, lipid droplets, lysosomes, microglia, mitochondria, quantum dots