Determination of single and competitive binary absorption isotherms for R and S optical isomers of fluoxetine
- UNCW Author/Contributor (non-UNCW co-authors, if there are any, appear on document)
- Jennifer L Beddoe (Creator)
- Institution
- The University of North Carolina Wilmington (UNCW )
- Web Site: http://library.uncw.edu/
- Advisor
- Dean Shirazi
Abstract: The pharmaceutical industry has come to rely on isotherm data when working with
preparative chromatography. The data derived during isotherm measurement is used to optimize
experimental conditions which will be used in the making of drugs.
For the R- and S- enantiomers of Fluoxetine, four different methods were used to
determine the single component isotherms and this data was fitted to the Langmuir Isotherm
Model. Both enantiomers, when tested singly, fit the model using the Frontal Analysis Method
and Retention Time Method, but did not fit the model when using the FACP and ECP methods.
The major objective of this research was to determine whether the single component
isotherm measurement can be used to predict the multi-component isotherm in order to avoid the
cumbersome, tedious and very time consuming procedures for measuring the multi-component
isotherm. Prediction of a multi-component isotherm using single component isotherms is in
most cases impossible due to the competition between the two solutes and the difference in
column saturation capacity of the two compounds. The two components of a chiral compound
have very similar physical properties and therefore they should have similar column saturation
capacities. The competitive binary isotherm was determined on the racemic mixture of
Fluoxetine using the Frontal Analysis Method for Multi-Component Isotherms. It was then
compared to values predicted from the Single Component studies and it was determined that the
Multi-Component isotherm could be predicted from data obtained from the isotherms of the
single components.
Determination of single and competitive binary absorption isotherms for R and S optical isomers of fluoxetine
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Created on 1/1/2009
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Additional Information
- Publication
- Thesis
- A Thesis Submitted to the University of North Carolina at Wilmington in Partial Fulfillment of the Requirement for the Degree of Masters of Science
- Language: English
- Date: 2009
- Keywords
- Fluoxetine--Therapeutic use, Fluoxetine--Analysis, Gas chromatography
- Subjects
- Fluoxetine -- Therapeutic use
- Gas chromatography
- Fluoxetine -- Analysis