Construction and fine-scale analysis of a high-density, genome-wide linkage map to examine meiotic recombination in the honey bee, Apis mellifera
- UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
- Katelyn Jo Miller (Creator)
- Institution
- The University of North Carolina at Greensboro (UNCG )
- Web Site: http://library.uncg.edu/
- Advisor
- Olav Rueppell
Abstract: The western honey bee, A. mellifera , is an important biological model organism in research for ecological and behavioral studies in addition to molecular studies. Honey bees are also imperative in nature for reproduction and diversification of plants via pollination. A unique feature of honey bees is that they have the highest recombination rate of all metazoans. This gives rise to the important question: what causes honey bees to have such a high rate of recombination? The honey bee genome has already been sequenced, but the available linkage maps are not detailed enough to characterize individual recombination events at the genome level. High recombination rates in honey bees may be caused by abundant recombination hotspots found throughout the genome. Resequencing the honey bee genome with next-generation sequencing and using over 900,000 markers genome-wide to identify recombination events showed that recombination rate in honey bees may be underestimated. This study calculated the average recombination rate to be 178.7 cM/Mb as opposed to the second most recent average of 22 cM/Mb. These high recombination rates in this study could be explained by mistakes in the current assembly of the reference genome. Further analyses are necessary to verify proper assembly of the current reference genome, genome-wide recombination events, and recombination rates. Based on the verified data set it will then be possible to confirm whether hotspots are present in honey bees and to correctly correlate recombination hotspots to sequence motifs.
Construction and fine-scale analysis of a high-density, genome-wide linkage map to examine meiotic recombination in the honey bee, Apis mellifera
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Created on 8/1/2014
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Additional Information
- Publication
- Thesis
- Language: English
- Date: 2014
- Keywords
- Honey bee, Apis mellifera, Recombination
- Subjects
- Honeybee $x Genetics
- Genetic recombination